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中国循证儿科杂志

中国循证儿科杂志 ›› 2019, Vol. 14 ›› Issue (6): 438-442.

• 论著 • 上一篇    下一篇

新生儿社区获得性耐甲氧西林金黄色葡萄球菌皮肤软组织感染临床分离株的分子、耐药性及毒力特征研究

耿文静1,李文婷2,史伟3,黑明燕1   

  1. 1 首都医科大学附属北京儿童医院新生儿中心 北京，100045 ; 2 山东大学齐鲁儿童医院 济南，250000；3 首都医科大学附属北京儿童医院、北京市儿科研究所，儿科学国家重点学科，教育部儿科重大疾病研究重点实验室，国家呼吸系统疾病临床医学研究中心 北京，100045
  • 收稿日期:2019-12-09 修回日期:2019-12-11 出版日期:2019-12-25 发布日期:2019-12-25
  • 通讯作者: 黑明燕
  • 基金资助:
     

Molecular characteristics of antibiotic resistance and virulence in community acquired methicillin-resistant Staphylococcus aureus from neonates with skin and soft tissue infections

GENG Wen-jing1, LI Wen-ting2, SHI Wei3, HEI Ming-yan1   

  1.  1 Neonatal Center, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China; 2 Respiratory Department, Qilu Children’s Hospital of Shandong University, Jinan 250000, China; 3 MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, China
  • Received:2019-12-09 Revised:2019-12-11 Online:2019-12-25 Published:2019-12-25
  • Contact: HEI Ming-yan
  • Supported by:
     

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摘要： 目的探讨新生儿社区获得性耐甲氧西林金黄色葡萄球菌（CA-MRSA）皮肤软组织感染临床分离株的分子、耐药性及毒力特征。方法从NICU中收集皮肤软组织感染的CA-MRSA菌株，总结其临床特点，对所有菌株进行MLST、SCCmec、SPA和agr分型，并行21种超抗原毒素基因、sasX基因和PVL基因检测。所有菌株进行体外药物敏感试验。结果28例CA-MRSA皮肤软组织感染的新生儿，单部位感染10例，多部位感染18例，检出30株CA-MRSA，以脐部蜂窝织炎为主（12/30,40.0%），主要的流行克隆为ST59-IVa-t437-agr-1（73%，22/30）, 超抗原毒力基因的携带率为80%，PVL的阳性率为47%。发现了4株苯唑西林敏感的MRSA（OS-MRSA），红霉素耐药率高。结论新生儿CA-MRSA皮肤软组织感染以脐部蜂窝织炎为主，主要的流行克隆为ST59-IVa-t437-agr-1, 超抗原及PVL基因携带率高，首次发现了OS-MRSA，红霉素耐药率高。

 

Abstract: ObjectiveTo study the characteristics of the molecular, drug resistance and virulence in community acquired methicillin-resistant staphylococcus aureus from neonates with skin and soft tissue infections. MethodsCA-MRSA isolates were prospectively collected from Beijing Children's Hospital. All the isolates were characterized by staphylococcal chromosomal cassette (SCCmec) type, staphylococcal protein A (spa) type and multilocus sequence type (MLST). Superantigen toxin gene, sasX gene, antimicrobial susceptibility were also detected. ResultsCellulitis of umbilicus was the most common case, and the most common CA-MRSA clones were ST59-SCCmecIVa-t437. The sasX gene was not detected. Four isolates were oxacillin-susceptible. ConclusionCellulitis of umbilicus was the most common case in neonates with skin and soft tissue infections, and ST59-SCCmecIVa-t437 was the most common clones. OS-MRSA was reported for the first time.

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