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Chinese Journal of Evidence -Based Pediatric ›› 2016, Vol. 11 ›› Issue (2): 109-112.

• Original Papers • Previous Articles     Next Articles

Clinical value of Mycoplasma pneumoniae RNA detection in the monitoring of Mycoplasma pneumoniae pneumonia treatment in children

GUO Li1,2,  SUN Lin1,GUO Yan1, LI Qin-jing1, WU Xi-rong1, LIU Fang1, LI Ying-jia1, WANG Xing-yun1, WANG Ting1, XU Bao-ping1, FENG Xue-li1, SHEN A-dong1   

  1. 1 Department of Respiratory, Beijing Children′s Hospital, Capital Medical University,  Beijing Key Laboratory of Pediatric Respiratory Infection Diseases; Key Laboratory of Major Diseases in Children and National Key Discipline of Pediatrics (Capital Medical University), Ministry of Education; National Clinical Research Center for Respiratory Diseases; Respiratory Infection Diseases Lab, Beijing Pediatric Research Institute, Beijing Children′s Hospital, Capital Medical University, Beijing, 100045; 2 Maternal and Child Health Care Center of Pinggu District; Beijing 101200, China
  • Received:2016-03-01 Revised:2016-04-21 Online:2016-04-25 Published:2016-04-21
  • Contact: SHEN A-dong

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Abstract:

Objective To evaluate the clinical utility of mycoplasma pneumonia RNA detection （real-time isothermal transcription-mediated RNA amplification assay, MP-SAT） in the treatment of Mycoplasma pneumoniae pneumonia in children. Methods Children with community-acquired pneumonia in Beijing Pinggu District Maternal and Child Health Care Hospital, according to the mycoplasma pneumoniae diagnostic criteria，were divided into the Mycoplasma pneumoniae pneumonia and non-Mycoplasma pneumoniae pneumonia groups. ①When the results of serological tests were considered as the standard，the sensitivity and specificity of MP-SAT were evaluated. ②MP - SAT and serum MP -Ab positive cases were observed dynamically，the detection rate of MP-SAT and MP-Ab in different treatment course and the times of MP-SAT turning to negative and clinical recovery were compared. ③The differences of fever duration after macrolide administration，blood routine inspection （WBC, N, L）, hypersensitive c-reactive protein (hs-CRP), serum lactate dehydrogenase (LDH) and the imaging results admitted to hospital within 24 hours were compared. Results Mycoplasma pneumoniae pneumonia group included 73 cases, non-Mycoplasma pneumoniae pneumonia group included 150 cases. The sensitivity, specificity of MP-SAT were 83.6%（61/73） and 97.3%（146/150）,respectively. Children with both MP-SAT and MP-Ab positive results were followed to evaluate the treatment effect. After the treatment, the detection rate of SAT reduced significantly (P<0.05), while detection rate of serum MP-Ab increased significantly (P<0.05). The time of clinical recovery was (3.0±0.9) weeks, similar to that of MP-SAT turning to negative（3.3±0.8) weeks (P＞0.05). Those with longer time of turning to negative had a longer fever duration after macrolide administration，increasing neutrophil，decreasing lymphocyte， higher level CRP and LDH, and large field of consolidation were more frequently observed on X-ray film (P<0.05). Conclusion MP-SAT can be used as an index for evaluation of the treatment outcome of MPP.