Abstract: Objective：To evaluate the effect of isothermal amplification chip method in lower respiratory tract pathogens detection. Methods：A total of 230 qualified sputum samples were collected. The pathogens were detected by using the isothermal amplification chip method and bacterial culture method. Mycobacterium tuberculosis was tested by using the Ziehl-Neelsen staining. Results：Pathogens were detected by using the isothermal amplification chip method in 182 specimens with the positive rate of 79.1%，which were mainly Methicillin-resistant staphylococcus aureus (33.8%), Haemophilus influenzae (27.7%), Streptococcus pneumoniae (22.5%). Of which, 77(33.5%) were single pathogen infection, 105(45.6%) were mixed infection and 48(20.9%) were pathogen negative. The positive rates of haemophilus influenzae, streptococcus pneumoniae, staphylococcus aureus and klebsiella pneumoniae detected using isothermal amplification chip were higher than culture. The total conformance rate of escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, and malt narrow-phagocytes by the two different methods were high. And the positive rate of mycoplasma pneumoniae detected by isothermal amplification chip (12.4%) was similar with that detected by argeted serological testing (11.8%). Conclusion：Isothermal amplification chip method is conducive to the rapid diagnosis of low respiratory tract infection of children and timely targeted treatment.